Autores: L. DNA (mtDNA) content remains unexplored. Brazil. Budowle, W. A non-radioactive dot-blot nucleic acid hybridization method was evaluated for detecting citrus leaf blotch virus (CLBV, Flexiviridae: Citrivirus). Hudlow. Using a CCD camera imaging system as a recording device to quantify human DNA by slot blot hybridization. Double-stranded mtDNA, estimated by slot-blot hybridization and real time PCR and expressed as mtDNA-to-nuclear DNA. Historical and commonly used quantitation methods include the following: Yield gels; Spectrophotometry; Fluorometry; Slot blot hybridization; AluQuant®. R. Klevan, B. . Dot-blot hybridization analysis of subtracted fragments membranes were screened by hybridization with genomic DNA from the tester (panel A) and the driver. In two, the source was unclear. Papillomavirus typing was carried out by HPV DNA dot and Southern blot hybridization using mixed HPV 6 11, 16 18, and 2 3 DNA. The majority of laboratories used the commercially available QuantiBlot® kit. On the other hand, PCR/ DBH was more sensitive than either PCR or. This DIGlabeled probe was capable of detecting viral copies of purified OvHV-2 DNA by DBH. RNA slot-blot hybridization of two cotton cultivars, Giza 45 and Giza 84, performed by use of their respective related probes G45 an d G84 probes. Slot blot hybridization was the most commonly used method until recently.