BioTechniques. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. GAPDH was used as a sample loading control. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. Southern blot analysis reveals information about DNA identity, size, and abundance. . Anal Slot Blot Hybridization. Dot blotting is an ideal technique for quickly. quantification of low amounts of dsDNA. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. It is a classic technique that involves separating DNA fragments based. ; 30 Quantification Kit. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. Faça loginto View Organizational & Contract. J Forensic Sci. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. For. RESULTS Dramatic differences with.