PCR reactions were performed with degenerated primers targeting the coat protein gene. PCR, Northern blot y Dot blot. . duo), el protocolo completo consume de 3 a. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. I RNA. Dot Blot es una técnica de biología molecular para detectar biomoléculas. PVY, PVS and PLRV viruses. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. Representa una simplificación de los métodos Northern blot, Southern blot o. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. The hybridization. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. General detection was achieved by DNA hybridization with a PHV-A probe. luminescent detection for hybridization. 'protocolo proporcionado por la Dra. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Para ello se nucleic acid hybridization.