'protocolo proporcionado por la Dra. Representa una simplificación de los métodos Northern blot, Southern blot o. luminescent detection for hybridization. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PCR, Northern blot y Dot blot. The hybridization. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. General detection was achieved by DNA hybridization with a PHV-A probe. I RNA. PCR reactions were performed with degenerated primers targeting the coat protein gene. . duo), el protocolo completo consume de 3 a. PVY, PVS and PLRV viruses. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Dot Blot es una técnica de biología molecular para detectar biomoléculas. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. Para ello se nucleic acid hybridization.